Czytniki luminescencji
- zakres długości fali 380nm - 630nm
- luminescencja flash ( z użyciem dozowników) i glow
- 2, 4 lub 12 dozowników
- odczyt płytek 96- i 384- dołkowych
- inkubator
- wytrząsanie
- oprogramowanie SoftMax Pro
Comparison of Microplate Readers with Luminescence Detection
|
Features |
SpectraMax |
Flex- |
||||
|
M3 |
M4 |
M5 |
M5e |
L |
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|
Detector |
Photo-multiplier tube |
Photo-multiplier tube |
Photo-multiplier tube |
Photo-multiplier tube |
1, 2, or 6 Ultra-fast Photomultiplier Tubes |
Photo-multiplier tube |
|
Spectral range |
250-850 |
250-850 |
250-850 |
250-850 |
380-630 nm |
250-850 |
|
nm |
nm |
nm |
nm |
|
nm |
|
|
Sensitivity |
<2 fg/well for firefly luciferase in 96- and 384- well top read |
<2 fg/well for firefly luciferase in 96- and 384- well top read |
<2 fg/well for firefly luciferase in 96- and 384- well top read |
<2 fg/well for firefly luciferase in 96- and 384- well top read |
20 attomol ATP per well, 0.2 fg firefly luciferase per well |
<2 fg/well for firefly luciferase in 96- and 384- well top read |
|
Dynamic range |
|
|
|
|
> 9 decades |
|
|
Microplate format |
6-384 |
6-384 |
6-384 |
6-384 |
96- and 384-well microplates |
6-384 (96- and 384-well with pipetting) |
|
Cross-talk |
<0.3% in white 96- and 384-well microplates |
<0.3% in white 96- and 384-well microplates |
<0.3% in white 96- and 384-well microplates |
<0.3% in white 96- and 384-well microplates |
< 3 x 10-5 (white plates) |
<0.3% in white 96- and 384-well microplates |
|
Read modes |
Endpoint, kinetic |
Endpoint, kinetic |
Endpoint, kinetic |
Endpoint, kinetic |
Endpoint, dual read, kinetic, fast kinetic |
Endpoint, kinetic, fast kinetic |
|
Read times |
0.1 up to 10 sec. integration time per well |
0.1 up to 10 sec. integration time per well |
0.1 up to 10 sec. integration time per well |
0.1 up to 10 sec. integration time per well |
0.1 up to 3600 sec. in steps of 0.1 sec. |
0.1 up to 10 sec. integration time per well |
|
Injectors/Pipettors |
N/A |
N/A |
N/A |
N/A |
2, 4, or 12 injectors |
8- or 16-channel pipettor |
|
Injection/pipettor volumes |
N/A |
N/A |
N/A |
N/A |
Selectable between 10 and 100 µl |
Selectable between 5-200 µL (96-well) and 1-25 µL (384) |
|
Temperature control |
X |
X |
X |
X |
X |
X |
|
Software brand |
SoftMax Pro |
SoftMax Pro |
SoftMax Pro |
SoftMax Pro |
SoftMax Pro |
SoftMax Pro |
|
Automatic waste disposal |
|
|
|
|
X |
|
|
Robotic/automation |
X |
X |
X |
X |
X |
X |
|
Validation & compliance tools |
X |
X |
X |
X |
X |
X |
Zapraszamy na stronę producenta o czytnikach luminescencji: http://www.moleculardevices.com/Products/Instruments/Microplate-Readers/Single-Mode-Readers/Luminescence.html